Some scanbox functions allow you to export *.sbx files to *.tif files that can be then visualized using other software like Vaa3d or ImageJ. For example, after collecting a z-stack you can use sbxtifstack() to generate a tif file that can be read directly using Vaa3d. If your stack has the hypothetical file name abc_010_nnn.sbx…
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Fast volumetric scanning with an electrically tunable lens
We have implemented volumetric scanning in Scanbox by means of Optotune‘s electrically tunable lens, which allows for fast changes in focal plane without moving parts. Some salient features of this particular implementation are: Laser power and focus can can co-vary, allowing the brightness of the images to remain approximately constant while performing volumetric imaging. This is achieved by…
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