Spatial Calibration for Multiple Objectives

Multiple users of the scope may be running projects that require different types of objectives.  How to keep a spatial calibration for each and switch between them when necessary? Scanbox now includes an “objective” configuration variable — a cell array of strings each with the name of a different objective.  Right now I have: Now,…

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Sub-pixel alignment in bidirectional scanning mode

When imaging at low magnification in bidirectional mode cells can be small, with a diameter of ~6 pixels or so.  In this and other situations the mechanism offer by the ncolbi variable to align even and odd lines may prove too coarse for optimal alignment. We added a new variable to the configuration file, named…

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Measuring the field of view and validating the uniformity of spatial correction

If you followed the instructions on spatial calibration you should have a nice uniform field in Scanbox.  One simple way to measure the resulting spatial resolution and spatial uniformity at each zoom setting is to mount a 40 line pairs per mm RONCHI calibration slide on top of a green aut0-fluorescent slide.  By aligning the…

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Bidirectional scanning and resonant driver calibration

Resonant mirrors vary somewhat in their resonant frequency and the settings of the mirror controller must be adjusted to obtain the best possible images in bidirectional mode. A synchronization signal from the resonant board must be adjusted so that it is both symmetric and its phase is aligned with the zero crossings of the mirror…

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Line scan and continuous resonant modes

The latest version of Scanbox includes two new features requested by users.  These appear are small toggle buttons on the left within the Scanner panel. The button on the bottom right of the panel is already known to you — it allows you to toggle between Unidirectional and Bidirectional scanning modes.  The letter shown in…

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Measuring the resonant frequency

The actual time a line takes to scan depends on the resonant frequency of the mirror.  This is typically around 8kHz but it can vary from one device to another.  One easy way to measure it is to split the signal that goes from TRIG OUT to the TRIG IN of the Alazartech board and feed…

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auto-tuning bidirectional scanning

To properly align even and odd lines in bidirectional tuning one has to set the values of the configuration variable ‘ncolbi’.  This variable is a vector where each entry controls how lines are ‘folded’ for each of the magnification settings, x1, x2 and x4. The latest version of scanbox includes a function sbxautocalbd() that allows you to automatically determine the…

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Larger field of view and finer control of pixel size

There have been requests for Scanbox to accommodate a larger field of view and the ability to precisely calibrate the aspect ratio and size of pixels.  The latest version (along with modifications in the firmware) allow you to do just this. [This paragraph about the diodes is only relevant to older boards] A first step to maximize…

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Bidirectional scanning in Scanbox

We have implemented bidirectional scanning to Scanbox. To switch between bidirectional and unidirectional scanning all you have to do is hit the toggle button within the “Scanner” panel. The default setting is “U”, standing for unidirectional scanning; hitting the button will switch it to “B”, indicating bidirectional scanning.  You can only switch between the two modes while the…

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