Multiple users of the scope may be running projects that require different types of objectives. How to keep a spatial calibration for each and switch between them when necessary? Scanbox now includes an “objective” configuration variable — a cell array of strings each with the name of a different objective. Right now I have: Now,…
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Spatial Calibration and return to origin
We added two new features to the knobby scheduler panel, as shown below by the arrows. One (left arrow) allows knobby to perform an automatic spatial calibration of the system, measuring the (x,y) size of pixels at all magnifications. To perform this calculation do the following: Focus on some pollen grains. Make sure one of the…
Read MoreUpdating the trinamic firmware
Some updates to Scanbox/Knobby require the update of the firmware in the Trinamic TMCM-6110 card inside the motor controller. To update the firmware do the following: Run the program yeti/drivers/TMCL.exe Select Setup->Options from the menu. In the connection tab, select the COM port that corresponds to the card (it is typically labeled “Stepper”), and Click Ok.…
Read MoreMeasuring the field of view and validating the uniformity of spatial correction
If you followed the instructions on spatial calibration you should have a nice uniform field in Scanbox. One simple way to measure the resulting spatial resolution and spatial uniformity at each zoom setting is to mount a 40 line pairs per mm RONCHI calibration slide on top of a green aut0-fluorescent slide. By aligning the…
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