There is one hidden feature of Scanbox that has been around for some time, but I have not yet described. This feature allows for computer-assisted segmentation during an experiment. As you must already know, one way in which region of interests (ROIs) can be defined is manually. This process can be initiated by clicking on the Add button…
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Quick start guide to the Optotune and volumetric scanning
Scanbox uses an on-board current source to control the Optotune lens, so that changes in depth are synchronized to the beginning of each microscope frame. Once the lens is installed, the easiest way to verify its function is to start imaging a sample and move the slider in the Optotune panel to change the plane of…
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