Intrinsic & epi-fluorescence imaging using the port camera

Some colleagues have asked about the possibility of integrating imaging acquisition from the port camera to do intrinsic and/or epi-fluorescence imaging ahead of targeting a region of interest with two-photon experiments in Scanbox. We have now added this option to Scanbox. To use this  option you will need to wire the I2C port on the…

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Fixing a jittery, linear actuator

Do you hear a high-pitched noise when the port camera mirror is in one of the opened and/or closed positions?  If so, it is possible the linear actuator is jittering a bit around its position. One way to solve this issue is to this install the Firgelli LAC configuration utility under yeti/firgelli.  Follow the instructions…

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Setting up the gige cameras

The following explains how to connect GigE cameras to your Scanbox.  If you have trouble following the instructions below just let me know — I can help remotely. ScanBox has the ability to acquire images from cameras synchronized to the frames of the microscope.  We typically use one camera to monitor eye movements and  a second one to monitor…

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