If you have one of the new systems (aka as the pizza box) you can now acquire imagery simultaneously from the port camera and the behavior cameras. During 2p imaging the behavior cameras are triggered at the beginning of a frame (TTL OUT 1x f.r.) or at the beginning and middle of a frame (TTL…
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Intrinsic & epi-fluorescence imaging using the port camera
Some colleagues have asked about the possibility of integrating imaging acquisition from the port camera to do intrinsic and/or epi-fluorescence imaging ahead of targeting a region of interest with two-photon experiments in Scanbox. We have now added this option to Scanbox. To use this option you will need to wire the I2C port on the…
Read MoreFixing a jittery, linear actuator
Do you hear a high-pitched noise when the port camera mirror is in one of the opened and/or closed positions? If so, it is possible the linear actuator is jittering a bit around its position. One way to solve this issue is to this install the Firgelli LAC configuration utility under yeti/firgelli. Follow the instructions…
Read MoreSetting up the gige cameras
The following explains how to connect GigE cameras to your Scanbox. If you have trouble following the instructions below just let me know — I can help remotely. ScanBox has the ability to acquire images from cameras synchronized to the frames of the microscope. We typically use one camera to monitor eye movements and a second one to monitor…
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