Multiple users of the scope may be running projects that require different types of objectives. How to keep a spatial calibration for each and switch between them when necessary? Scanbox now includes an “objective” configuration variable — a cell array of strings each with the name of a different objective. Right now I have: Now,…
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Sub-pixel alignment in bidirectional scanning mode
When imaging at low magnification in bidirectional mode cells can be small, with a diameter of ~6 pixels or so. In this and other situations the mechanism offer by the ncolbi variable to align even and odd lines may prove too coarse for optimal alignment. We added a new variable to the configuration file, named…
Read MoreMeasuring the field of view and validating the uniformity of spatial correction
If you followed the instructions on spatial calibration you should have a nice uniform field in Scanbox. One simple way to measure the resulting spatial resolution and spatial uniformity at each zoom setting is to mount a 40 line pairs per mm RONCHI calibration slide on top of a green aut0-fluorescent slide. By aligning the…
Read MoreBidirectional scanning and resonant driver calibration
Resonant mirrors vary somewhat in their resonant frequency and the settings of the mirror controller must be adjusted to obtain the best possible images in bidirectional mode. A synchronization signal from the resonant board must be adjusted so that it is both symmetric and its phase is aligned with the zero crossings of the mirror…
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