If you followed the instructions on spatial calibration you should have a nice uniform field in Scanbox. One simple way to measure the resulting spatial resolution and spatial uniformity at each zoom setting is to mount a 40 line pairs per mm RONCHI calibration slide on top of a green aut0-fluorescent slide. By aligning the…
Read MoreAll articles filed in April 2016
Auto calibration of SLM and two-photon paths
You’ve already heard about out plan to add an SLM path to the NLW two-photon microscope. As we described earlier the first step is to provide a tool to align the SLM and 2P paths. This is now complete and it consists of four phases carried out automatically by a Matlab function: A port camera connected to…
Read MoreA simple lick-o-meter and liquid reward delivery system
Some behavioral experiments reward correct performance. A typical response is a lick of a spout and the reward is a fixed volume of water. One simple way to achieve this is by using an Arduino board along with the capacitive sensing library. The diagram below summarizes the parts used. Just connect a 1 Mohm resistor between pins…
Read MoreUpgrading to Windows 10
Scanbox performance is better in Windows 10. We recommend the upgrade in general and highly if you use bidirectional scanning frequently. To upgrade, follow these steps. Upgrade to Windows 10. Make a fresh install of the lastest nVidia driver for your board. Install the latest 3D connexion driver. Install the latest AlazarTech driver for the 9440…
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