Optogenetics with Scanbox

The latest version of Scanbox includes a panel to allow uses to perform 1p optogenetics with 2p imaging.  The panel provides an interface to the ThorLabs DC2200 dual channel controller, but can also be used with the simpler T-cube driver.  You can use the LED of your preference.  We have been working both with mounted…

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Network Commands

The basics of how to control the microscope over the network have been described here. Over time, a number of other commands have been added to facilitate the control of Scanbox remotely.  Here is an up-to-date list of network commands.  If there are others you need just let us know. File storage: A<string> Set animal…

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Using an auxiliary USB DAQ to sample TTL events

We have added an option to sample an additional 8 TTL inputs from Scanbox using an external NI DAQ device.  In our case, we have used and tested the NI USB-6221, but any other devices which support sampled, digital IO should work as well. The configuration file now contains four lines that specifies the parameters…

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Support for Phenosys Speedbelt Linear Treadmill

Scanbox now provides a direct interface to Phenosys’ speedbelt.  Once the speedbelt is connected via the USB to the computer it will show up as a COM port on the Device Manager.  To acquire input from the treadmill, simply define the speedbelt_com configuration variable specifying the corresponding COM port. Within the Scanbox “Treadmill and Encoder”…

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Motor box update

We are asking all Scanbox users to update to the latest version of Scanbox which incorporates important updates to the motor box code, including: Update of the Trinamic firmware Update of the motor box application code Update of the motor box interface code in Scanbox Please proceed as follows: If you don’t have the Trinamic…

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Happy Holidays from Scanbox

Happy holidays to all Scanbox users! This was a very productive year.  We introduced a number of new features including: Introduction of Virtual Knobby Introduction of Knobby 2.0 Introduction of Knobby Scheduler Support for TTL start/stop acquisition mode Enhancements to alignment and segmentation tools Introduction of the plugin server Introduced spatial calibration and return to origin…

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Automatic Pockels Linearization

Some time ago, we described a procedure to manually create a look up table for the laser power slider to achieve linear power.  Now, if you have a Thorlabs’ PM100D meter your job has been made much easier, as Scanbox can perform the calibration automatically. As a first step, you will need download and install the…

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Finding the optimal sample clock phase

Some time ago we talked about the advantages of synchronizing the digitizer sample clock to the laser.  Our new system incorporates an on-board PLL multi-phase clock that allows Scanbox to automatically measure the contrast in a test image as a function of sample phase delay and select the optimal value for your setup. A typical measurement…

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Using the correct range and bias for PMT amplifiers

You may have one of two different PMT amplifiers in your setup.  One which has a variable gain. The typical settings of this amplifier should be: left the switch on GND, middle switch on L or H but with the knob set to a gain of 10^4, next switch on FBW and DC for the…

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Taking the mesoscope for a test drive

The Neurolabware mesoscope is up and running using our new tower system and today we took it for our first test drive with a tetO mouse. Here is the very first movie sequence of a large FOV with single-cell resolution.   We can also build a panorama of a large region using Lissajous scanning (other…

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