Non-rigid deformation for calcium imaging frame alignment

xcorr: comp neuro

Oh yeah!

It’s been a while since I last updated the blog – I graduated from McGill, went on a trip to Indonesia where I did a lot of diving (above), came back to Montreal for 16 hours to gather my coffee machine and about three shirts – all I need to survive, really – then moved to sunny California to do a postdoc in Dr. Dario Ringach’s lab at UCLA. Living the dream.

We’ve been doing calcium imaging – GCamp6 – in mice via a custom-built microscope – you can read more about the microscope over at the Scanbox blog.

If you’re used to working with single electrode or even MUAs, calcium imaging will blow your freaking mind – you can get more than a hundred shiny neurons in a given field of view, so you can realistically ask and answer questions about how a local circuit works knowing that you’re not massively undersampling the population.

Inter-frame motion is an issue in awake…

View original post 515 more words