We are preparing Scanbox for fast, volumetric scanning, by providing simultaneous control of an electrically tunable lens (to focus on different planes) and a Pockels cell (to control laser power).
The Scanbox card has an integrated, 12-bit current source used to control the focus tunable lens. Similarly, an integrated DAC on the Scanbox card controls laser power via a Pockels cell. The mechanism implemented still allows for user-defined blanking of the margins. Arbitrary tables can be uploaded from the Scanbox GUI to the card to allow for simultaneous changes in these variables that are precisely synchronized to the frames of the microscope.
Below is a demonstration showing a sinusoidal change in focusing along with matching changes in laser power. Fast scanning with synchronized depth/power control is coming to Yeti soon![vimeo 119605807 w=500&h=280]