Using USB3 cameras with Scanbox

Scanbox has long supported the use of GigE cameras for behavior, but integrating USB3 cameras which support an external trigger is also possible.  As an example, consider the Mako-U series. Wire up the camera To use one of these cameras you have to be using a trigger cable.  Different manufacturers offer cables that differ in…

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Simultaneous imaging with port and behavior cameras

If you have one of the new systems (aka as the pizza box) you can now acquire imagery simultaneously from the port camera and the behavior cameras. During 2p imaging the behavior cameras are triggered at the beginning of a frame (TTL OUT 1x f.r.) or at the beginning and middle of a frame (TTL…

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A simple lick-o-meter and liquid reward delivery system

Some behavioral experiments reward correct performance. A typical response is a lick of a spout and the reward is a fixed volume of water. One simple way to achieve this is by using an Arduino board along with the capacitive sensing library.  The diagram below summarizes the parts used.  Just connect a 1 Mohm resistor between pins…

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Using the quadrature encoder to track locomotion

Scanbox allows you to acquire the position of a rotating platform at each frame of the microscope.  To do so, you need a quadrature encoder to be mounted on the axis of your platform.  We use the US Digital HS-360-IE-S, but any quadrature encoder will do as well. The encoder needs to be wired to an Arduino…

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Blanking the laser between trials

Sometimes, during long behavioral sessions, it is advantageous to turn off the laser between trials to minimize damage while keeping the microscope scanning.  To do this without introducing any additional noise that may influence the behavioral state we use the Pockels cell.  Scanbox now allows one to blank the laser by sending a command over…

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