The Mesoscope Panel

If you are already familiar with Scanbox on the NLW standard system, you will find it very natural to use the new options available in the mesoscope. When Scanbox is configured to operate the mesoscope, you will find a new panel giving access to the features that go beyond those of the standard configuration: Here is…

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Measuring the field of view and validating the uniformity of spatial correction

If you followed the instructions on spatial calibration you should have a nice uniform field in Scanbox.  One simple way to measure the resulting spatial resolution and spatial uniformity at each zoom setting is to mount a 40 line pairs per mm RONCHI calibration slide on top of a green aut0-fluorescent slide.  By aligning the…

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Simultaneous imaging and electrophysiology

Scanbox now allows the possibility of doing simultaneous imaging and electrophysiology.  An external DAQ device, supported by Matlab’s data acquisition toolbox, is need to do this.  In our case, we had an old NI-USB-6221 lying around that we decided to use. In the present case, we set things up to do patch-clamp recordings from a single…

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