Sub-pixel alignment in bidirectional scanning mode

When imaging at low magnification in bidirectional mode cells can be small, with a diameter of ~6 pixels or so.  In this and other situations the mechanism offer by the ncolbi variable to align even and odd lines may prove too coarse for optimal alignment. We added a new variable to the configuration file, named…

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Improvements in alignment and segmentation tools

Two GUIs to align (sbxaligntool) and segment (sbxsegmenttool) two-photon imaging data from Scanbox were recently updated. Sbxaligntool allows users to perform alignment using rigid translation or a non-rigid deformation of the image stack. The tool now uses all the cores available in your computer along with the GPU.  Rigid alignment can take place at near…

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basic processing pipeline

Scanbox includes some basic processing functions that you can use to (a) align the images to compensate for (x,y) movement, (b) segment cells, (c) extract the signals corresponding to the segmented cells from the image sequences. Motion compensation (sbxalignx & sbxaligndir) The first step that is sometimes required is to compensate for motion in the…

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Recursive Image Alignment and Statistics

If it is awake, it will move.  And if it moves, your images are likely to do it too.  This means that, almost surely, the first step in processing your data will be to align (or register) all the images in the sequence. This is such a basic problem that there are probably as many solutions…

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