Some experiments require long imaging times. In cases where we are recording from small structures, such as dendritic processes or spines, any slow drift in the depth of the imaging plane would affect the quality of the data. Here is a Scanbox plugin that will: (a) perform a quick z-stack around the desired imaging plane,…
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Real-time, motion compensation in Scanbox
Our colleague Tobias Rose was recently asking about Scanbox’s ability to stabilize the motion of images and signals from regions-of-intrest (ROIs) in real-time. The goal of such processing is to be able to do experiments in closed-loop, and do very quick analyses on the neuronal responses, such as computing tuning curves on the fly. Below is a…
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