Some experiments require long imaging times. In cases where we are recording from small structures, such as dendritic processes or spines, any slow drift in the depth of the imaging plane would affect the quality of the data. Here is a Scanbox plugin that will: (a) perform a quick z-stack around the desired imaging plane,…
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Using auto-stabilization
Real-time auto-stabilization in Scanbox Yeti is achieved by tracking the displacement of 2D features in a number of small windows relative to a reference image. We prefer tracking cells within a handful of small windows because slow gradients in the image can easily bias the resulting estimates for large regions. Here are some step-by-step instructions on how…
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Scanbox 