Measuring the field of view and validating the uniformity of spatial correction

If you followed the instructions on spatial calibration you should have a nice uniform field in Scanbox.  One simple way to measure the resulting spatial resolution and spatial uniformity at each zoom setting is to mount a 40 line pairs per mm RONCHI calibration slide on top of a green aut0-fluorescent slide.  By aligning the…

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Reading raw data with sbxread()

Raw image data in scanbox is contained in *.sbx files.  To read these data you can use the sbxread() function. Here is the documentation from its help section– So, for example, the following lines of code will read images from 0 to 19 (20 total) and display the average of the first (green) PMT channel: And you may…

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