Automatic Control of Laser Power

A new checkbox within the Laser panel (labeled AGC) allows you to turn an automatic control of laser power on and off. When AGC is on, Scanbox checks the distribution of pixel values on the image every T seconds, and increases or decreases the laser power by a certain factor if the fraction of pixels…

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Automatic Optotune Calibration

We previously explained how to calibrate the optotune manually.  With the introduction of Knobby 2, we are able to make this process automatic.  You will now find a ‘Calibration’ button in the Optotune panel.  To use it, do the following: Set the optotune slider to its lowest value (slide all the way down) Bring some pollen…

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Spatial Calibration and return to origin

We added two new features to the knobby scheduler panel, as shown below by the arrows. One (left arrow) allows knobby to perform an automatic spatial calibration of the system, measuring the (x,y) size of pixels at all magnifications.  To perform this calculation do the following: Focus on some pollen grains.  Make sure one of the…

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Knobby improvements

We have added a couple of new features to Knobby based on user feedback that some may find useful. These features are presented in different screen pages. You can switch between the three available pages by touching one of the 3 gray disks on the bottom left of the screen. The first screen is the…

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