Spatial Calibration for Multiple Objectives

Multiple users of the scope may be running projects that require different types of objectives.  How to keep a spatial calibration for each and switch between them when necessary? Scanbox now includes an “objective” configuration variable — a cell array of strings each with the name of a different objective.  Right now I have: Now,…

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Automatic Optotune Calibration

We previously explained how to calibrate the optotune manually.  With the introduction of Knobby 2, we are able to make this process automatic.  You will now find a ‘Calibration’ button in the Optotune panel.  To use it, do the following: Set the optotune slider to its lowest value (slide all the way down) Bring some pollen…

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Click-and-Center with Knobby Mouse Control

If you have been making use of Knobby’s spatial calibration button, you can now move on to interacting with Knobby remotely. The new Knobby scheduler panel looks a bit expanded.  The speed (coarse/fine/super-fine), the mode (normal/rotate), and zero (XYZ/XYZA) buttons should be self explanatory. They do exactly the same as if you were to be…

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Spatial Calibration and return to origin

We added two new features to the knobby scheduler panel, as shown below by the arrows. One (left arrow) allows knobby to perform an automatic spatial calibration of the system, measuring the (x,y) size of pixels at all magnifications.  To perform this calculation do the following: Focus on some pollen grains.  Make sure one of the…

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Knobby scheduler

A new Scanbox panel allows users to define arbitrary changes in (x,y,z) position over time (frames) which are then executed by Knobby (version 2 only) while imaging. Each entry define changes in x, y and z (in micrometers) relative to the present position and the frame number at which they will take place. The “mem”…

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Knobby 2.0

  Have you ever had trouble hitting the menu items in Knobby? Do you have big, fat fingers like Josh or Dario? Then Knobby 2.0 is for you! Featuring a 5″ diagonal touch screen with real buttons and added functionality! Coming to you soon…  Stay tuned!

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Virtual Knobby

Happy new year! We have plenty of exciting Scanbox developments happening this year, so stay tuned to the blog.  You don’t want to miss anything! We recently introduced a wireless version of knobby that runs on Android tablets. The same software is now available to run on Windows, side-by-side your Scanbox application. The controls and behavior…

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Disabling Knobby’s automatic reset

[Note: please do not use this feature yet — there are some bugs that need to be worked out. I will keep you posted.] Up to now starting a new Scanbox session from Matlab causes knobby will auto-reset, bringing all the position counters back to zero and clearing all its position memories. This is done because…

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Knobby improvements

We have added a couple of new features to Knobby based on user feedback that some may find useful. These features are presented in different screen pages. You can switch between the three available pages by touching one of the 3 gray disks on the bottom left of the screen. The first screen is the…

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Updating the trinamic firmware

Some updates to Scanbox/Knobby require the update of the firmware in the Trinamic TMCM-6110 card inside the motor controller.  To update the firmware do the following: Run the program yeti/drivers/TMCL.exe Select Setup->Options from the menu. In the connection tab, select the COM port that corresponds to the card (it is typically labeled “Stepper”), and Click Ok.…

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