Visualizing Individual Slices during Volumetic Imaging

During volumetric imaging, Scanbox displays all images as they are acquired. This can be inconvenient if we are trying to visually assess the activity of neurons within any one optical slice.  One solution is to display the images separately in a montage by means of a Scanbox plug-in.  However, it would be better to have…

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Automatic Control of Laser Power

A new checkbox within the Laser panel (labeled AGC) allows you to turn an automatic control of laser power on and off. When AGC is on, Scanbox checks the distribution of pixel values on the image every T seconds, and increases or decreases the laser power by a certain factor if the fraction of pixels…

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Automatic Optotune Calibration

We previously explained how to calibrate the optotune manually.  With the introduction of Knobby 2, we are able to make this process automatic.  You will now find a ‘Calibration’ button in the Optotune panel.  To use it, do the following: Set the optotune slider to its lowest value (slide all the way down) Bring some pollen…

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A Plug-in Server

Scanbox has a memory mapped mechanism to share the incoming data stream with other processes that intend to consume the data in real-time. Examples of simple uses of such data sharing include the calculation of rolling averages, displaying a real-time histogram, or generating a montage display for volumetric data. Of course, different experiments may need…

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New alignment and segmentation tools

Improved alignment and segmentation tools have now been released in the latest version of Scanbox, while retaining much of the functionality of the last version. sbxaligntool. The new alignment tool, shown below, adds batch processing of files, including the processing of eye and ball motion if those data are present.  A region-of-interest (ROI) can optionally be…

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Knobby scheduler

A new Scanbox panel allows users to define arbitrary changes in (x,y,z) position over time (frames) which are then executed by Knobby (version 2 only) while imaging. Each entry define changes in x, y and z (in micrometers) relative to the present position and the frame number at which they will take place. The “mem”…

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Bada boom! New system coming soon!

So why the long silence in the Scanbox blog? We have been working hard on a the development of our new system. A modular, expandable system that will run the new line of Neurolabware microscopes (aka the Kraken microscope) and is backward compatible with our previous box. Want a sneak peak? Bada boom!   Here is…

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Virtual Knobby

Happy new year! We have plenty of exciting Scanbox developments happening this year, so stay tuned to the blog.  You don’t want to miss anything! We recently introduced a wireless version of knobby that runs on Android tablets. The same software is now available to run on Windows, side-by-side your Scanbox application. The controls and behavior…

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