Scanbox is almost ready for in-vivo, two photon stimulation using spatial light modulators! In our first set of experiments we are are testing the system in SOM cells co-expressing C1V1 and GCaMP6. Prior to each experiment, we perform a calibration to ensure alignment of the SLM and imaging paths (takes ~3 min). Cells for stimulation are selected in the same fashion as we define ROIs for…
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Auto calibration of SLM and two-photon paths
You’ve already heard about out plan to add an SLM path to the NLW two-photon microscope. As we described earlier the first step is to provide a tool to align the SLM and 2P paths. This is now complete and it consists of four phases carried out automatically by a Matlab function: A port camera connected to…
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