If you have been making use of Knobby’s spatial calibration button, you can now move on to interacting with Knobby remotely. The new Knobby scheduler panel looks a bit expanded. The speed (coarse/fine/super-fine), the mode (normal/rotate), and zero (XYZ/XYZA) buttons should be self explanatory. They do exactly the same as if you were to be…
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Spatial Calibration and return to origin
We added two new features to the knobby scheduler panel, as shown below by the arrows. One (left arrow) allows knobby to perform an automatic spatial calibration of the system, measuring the (x,y) size of pixels at all magnifications. To perform this calculation do the following: Focus on some pollen grains. Make sure one of the…
Read MoreA Plug-in Server
Scanbox has a memory mapped mechanism to share the incoming data stream with other processes that intend to consume the data in real-time. Examples of simple uses of such data sharing include the calculation of rolling averages, displaying a real-time histogram, or generating a montage display for volumetric data. Of course, different experiments may need…
Read MoreNew alignment and segmentation tools
Improved alignment and segmentation tools have now been released in the latest version of Scanbox, while retaining much of the functionality of the last version. sbxaligntool. The new alignment tool, shown below, adds batch processing of files, including the processing of eye and ball motion if those data are present. A region-of-interest (ROI) can optionally be…
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Spikefinder: the Vanilla algorithm
Over the last few months, the Spikefinder challenge has provided a playing ground for colleagues to offer ideas about how to estimate the spiking of individual neurons based on the measured activity of fluorescent calcium indicators. The challenge was for people to come up with strategies that beat the performance of state-of-the-art algorithms, STM & oopsi. A good number of algorithms were able…
Read MoreExternal TTL trigger
To automatically start /stop acquisition by means of an external TTL signal follow the following instructions. First, connect an appropriate TTL control signal to the P1.6 (pin #21) of the extension header of the Scanbox board. The view below shows a top view of the Scanbox control board. The pin in question is located on…
Read MoreKnobby scheduler
A new Scanbox panel allows users to define arbitrary changes in (x,y,z) position over time (frames) which are then executed by Knobby (version 2 only) while imaging. Each entry define changes in x, y and z (in micrometers) relative to the present position and the frame number at which they will take place. The “mem”…
Read MoreKnobby 2.0
Have you ever had trouble hitting the menu items in Knobby? Do you have big, fat fingers like Josh or Dario? Then Knobby 2.0 is for you! Featuring a 5″ diagonal touch screen with real buttons and added functionality! Coming to you soon… Stay tuned!
Read MoreMesoscope almost ready!
Get ready for larger windows…
Read MoreHow to update Scanbox?
How to update Scanbox
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