Surface sampling in Scanbox
Answering a request from colleagues in London, the upcoming version of the Scanbox firmware will allow users to change the depth of imaging on a line-by-line basis. Up to now, during volumetric imaging, users were allowed to change the setting of the electronically tunable lens (ETL) once per frame (during the transition from one frame to another). The…
Read MoreScanbox and me on a boring afternoon
What do you do on a boring afternoon with a two-photon microscope? Looking at small stuff of course! All these guys are from the Carolina mixed pollen grains slide.
Read MoreDisabling Knobby’s automatic reset
[Note: please do not use this feature yet — there are some bugs that need to be worked out. I will keep you posted.] Up to now starting a new Scanbox session from Matlab causes knobby will auto-reset, bringing all the position counters back to zero and clearing all its position memories. This is done because…
Read MoreNew Features in Scanbox 3.4
The latest release adds a few new features and fixes. A new panel called “Deadband & Alignment” offers the option to change some of the configuration variables on the fly. The deadband sliders allow to dynamically change the size of the left and right margins respectively, where the laser is blanked out. You can optionally…
Read MoreInstalling knobby tablet
Before you start you will need an android tablet and a wireless router in the Lab. I have a Samsung Galaxy Tab E and an Airport Express, but other tablets and routers should work as well. To install knobby tablet follow the following steps: Download knobby tablet from the Google Play store. Download and install the…
Read MoreKnobby tablet is on the Google Play Store
You can download it on any Android tablet and give it a try. I will explain how to set things up in detail after I return from SfN. In the Lab, your tablet will need to be on the same wireless network as the desktop computer running Scanbox. If that computer does not have a wireless…
Read MoreKnobby gets a wireless sister
A welcome addition to the family: Knobby Tablet. Works on any Android tablet. Makes it easier to move the microscope from anywhere in the Lab. More details soon!
Read MoreSub-pixel alignment in bidirectional scanning mode
When imaging at low magnification in bidirectional mode cells can be small, with a diameter of ~6 pixels or so. In this and other situations the mechanism offer by the ncolbi variable to align even and odd lines may prove too coarse for optimal alignment. We added a new variable to the configuration file, named…
Read MoreImportant enhancements in Scanbox 3.3 firmware
Good news! The latest Scanbox version in Github includes a few important improvements and fixes: The new firmware substantially decreases line jitter producing even shaper images. It removes the artifactual horizontal lines from the mean of bidirectional scanning acquisitions. It fixes a bug in the phase of the camera trigger signal. It checks for cases where no…
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