Scanbox is almost ready for in-vivo, two photon stimulation using spatial light modulators! In our first set of experiments we are are testing the system in SOM cells co-expressing C1V1 and GCaMP6. Prior to each experiment, we perform a calibration to ensure alignment of the SLM and imaging paths (takes ~3 min). Cells for stimulation are selected in the same fashion as we define ROIs for…
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Adding an SLM path for optogenetics
As promised, there has been some progress in adding an SLM path for optogenetics to the microscope and things are looking good… We are now calling on potential users to provide input into the features that they would need/want in a GUI interface, as this may impact how useful this addition will be to our own…
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