All articles filed in two-photon stimulation

Scanbox is almost ready for in-vivo, two photon stimulation using spatial light modulators!  In our first set of experiments we are are testing the system in SOM cells co-expressing C1V1 and GCaMP6.  Prior to each experiment, we perform a calibration to ensure alignment of the SLM and imaging paths (takes ~3 min).  Cells for stimulation are selected in the same fashion as we define ROIs for…