Optogenetics with Scanbox

The latest version of Scanbox includes a panel to allow uses to perform 1p optogenetics with 2p imaging.  The panel provides an interface to the ThorLabs DC2200 dual channel controller, but can also be used with the simpler T-cube driver.  You can use the LED of your preference.  We have been working both with mounted…

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Scanbox does 2p-SLM stimulation! (And the mystery of the disappearing cell)

Scanbox is almost ready for in-vivo, two photon stimulation using spatial light modulators!  In our first set of experiments we are are testing the system in SOM cells co-expressing C1V1 and GCaMP6.  Prior to each experiment, we perform a calibration to ensure alignment of the SLM and imaging paths (takes ~3 min).  Cells for stimulation are selected in the same fashion as we define ROIs for…

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