Here is a description of some recent features added to facilitated patterned photo-stimulation experiments. The first step, of course, is the definition of ROIs (or masks) to stimulate. After collecting some data from one field of view, you can either use the “Segment” button to have Suite2p detect the cells or manually draw them by…
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Optogenetics with the Tower
The optogenetics panel in the new Tower electronics (aka the Pizza Box) is nearly the same as its predecessor. The main difference is that synchronization and timing of stimulation is done internally and does not require any external electronics. The parameters at the bottom specify the width of the LED, GATE pulses and the number…
Read MoreOptogenetics with Scanbox
The latest version of Scanbox includes a panel to allow uses to perform 1p optogenetics with 2p imaging. The panel provides an interface to the ThorLabs DC2200 dual channel controller, but can also be used with the simpler T-cube driver. You can use the LED of your preference. We have been working both with mounted…
Read MoreScanbox does 2p-SLM stimulation! (And the mystery of the disappearing cell)
Scanbox is almost ready for in-vivo, two photon stimulation using spatial light modulators! In our first set of experiments we are are testing the system in SOM cells co-expressing C1V1 and GCaMP6. Prior to each experiment, we perform a calibration to ensure alignment of the SLM and imaging paths (takes ~3 min). Cells for stimulation are selected in the same fashion as we define ROIs for…
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