Automatic Optotune Calibration

We previously explained how to calibrate the optotune manually.  With the introduction of Knobby 2, we are able to make this process automatic.  You will now find a ‘Calibration’ button in the Optotune panel.  To use it, do the following: Set the optotune slider to its lowest value (slide all the way down) Bring some pollen…

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Click-and-Center with Knobby Mouse Control

If you have been making use of Knobby’s spatial calibration button, you can now move on to interacting with Knobby remotely. The new Knobby scheduler panel looks a bit expanded.  The speed (coarse/fine/super-fine), the mode (normal/rotate), and zero (XYZ/XYZA) buttons should be self explanatory. They do exactly the same as if you were to be…

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Spatial Calibration and return to origin

We added two new features to the knobby scheduler panel, as shown below by the arrows. One (left arrow) allows knobby to perform an automatic spatial calibration of the system, measuring the (x,y) size of pixels at all magnifications.  To perform this calculation do the following: Focus on some pollen grains.  Make sure one of the…

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A Plug-in Server

Scanbox has a memory mapped mechanism to share the incoming data stream with other processes that intend to consume the data in real-time. Examples of simple uses of such data sharing include the calculation of rolling averages, displaying a real-time histogram, or generating a montage display for volumetric data. Of course, different experiments may need…

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New alignment and segmentation tools

Improved alignment and segmentation tools have now been released in the latest version of Scanbox, while retaining much of the functionality of the last version. sbxaligntool. The new alignment tool, shown below, adds batch processing of files, including the processing of eye and ball motion if those data are present.  A region-of-interest (ROI) can optionally be…

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External TTL trigger

To automatically start /stop acquisition by means of an external TTL signal follow the following instructions. First, connect an appropriate TTL control signal to the P1.6 (pin #21) of the extension header of the Scanbox board.  The view below shows a top view of the Scanbox control board.  The pin in question is located on…

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Knobby scheduler

A new Scanbox panel allows users to define arbitrary changes in (x,y,z) position over time (frames) which are then executed by Knobby (version 2 only) while imaging. Each entry define changes in x, y and z (in micrometers) relative to the present position and the frame number at which they will take place. The “mem”…

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Knobby 2.0

  Have you ever had trouble hitting the menu items in Knobby? Do you have big, fat fingers like Josh or Dario? Then Knobby 2.0 is for you! Featuring a 5″ diagonal touch screen with real buttons and added functionality! Coming to you soon…  Stay tuned!

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