We have added an option to sample an additional 8 TTL inputs from Scanbox using an external NI DAQ device. In our case, we have used and tested the NI USB-6221, but any other devices which support sampled, digital IO should work as well. The configuration file now contains four lines that specifies the parameters…
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Support for Phenosys Speedbelt Linear Treadmill
Scanbox now provides a direct interface to Phenosys’ speedbelt. Once the speedbelt is connected via the USB to the computer it will show up as a COM port on the Device Manager. To acquire input from the treadmill, simply define the speedbelt_com configuration variable specifying the corresponding COM port. Within the Scanbox “Treadmill and Encoder”…
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Motor box update
We are asking all Scanbox users to update to the latest version of Scanbox which incorporates important updates to the motor box code, including: Update of the Trinamic firmware Update of the motor box application code Update of the motor box interface code in Scanbox Please proceed as follows: If you don’t have the Trinamic…
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Mesoscope features: real-time panoramas, ROI selection, zoom/pan, and dynamic ROIs
Here are some of the features we have been developing to make it easy to build panoramas, pick regions-of-interest, and to visualize ROI location and data during acquisition using the NLW Mesoscope (aka the Kraken) on the latest version of Scanbox. The video below shows a brief demo of the following: The automatic creation of…
Read MoreFinding the optimal sample clock phase
Some time ago we talked about the advantages of synchronizing the digitizer sample clock to the laser. Our new system incorporates an on-board PLL multi-phase clock that allows Scanbox to automatically measure the contrast in a test image as a function of sample phase delay and select the optimal value for your setup. A typical measurement…
Read MoreUsing the correct range and bias for PMT amplifiers
Update[6/1/2018]: Please update Scanbox to make this feature work correctly. An error in the code of earlier versions incorrectly set the gain for fixed-type amplifiers (variable type amplifiers were handled correctly). An alternative, easy way to fix the issue without having to update Scanbox is to search for the constant INPUT_RANGE_PM_1V in scanbox.m and replace…
Read MoreTaking the mesoscope for a test drive
The Neurolabware mesoscope is up and running using our new tower system and today we took it for our first test drive with a tetO mouse. Here is the very first movie sequence of a large FOV with single-cell resolution. We can also build a panorama of a large region using Lissajous scanning (other…
Read MoreIntrinsic & epi-fluorescence imaging using the port camera
Some colleagues have asked about the possibility of integrating imaging acquisition from the port camera to do intrinsic and/or epi-fluorescence imaging ahead of targeting a region of interest with two-photon experiments in Scanbox. We have now added this option to Scanbox. To use this option you will need to wire the I2C port on the…
Read MoreSampling on a Surface
Not long ago I mentioned Scanbox’s new ability to sample on a surface. Now you can access this new feature in the GUI by navigating to the Surface Sampling panel. Surface sampling allows you to link lines of the resonant scan to depths determined by the optotune setting. In other words, it allows you to…
Read MoreTiling with Knobby
Knobby scheduler allows the user to move the microscope by specifying a list of relative changes in position at given frame numbers. One quick way to fill up the table is provided by a set of text fields in the knobby panel. This allows one to perform (x,y,z) tiling with a given range and step…
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